PCR-diagnosis of infections and the scope of its use

Human health depends on his way of life by as much as 50%. This includes factors such as personal hygiene, nutrition, the regime of the day, as well as the presence or absence of bad habits. However, no one has managed to maintain absolute health for many years, and even the apparent satisfactory state can not be the guarantor of such a state.

Development of medicine

In this regard, along with the importance of disease prevention, an increasingly important role is played by their maximally early detection. This contribution is made by the newest biotechnologies, which create a revolution in this sphere. This includes computer and magnetic resonance imaging, and PCR diagnostics of infections, and nuclear magnetic resonance spectroscopy, and even the construction of virtual 3D models of various organs and parts of the human body. Thanks to the development of science, medicine does not stand still, opening a completely different level of health care for patients.

Relevance

As already mentioned, PCR diagnostics of infections in its time simply exploded the microbiological world and showed new possibilities for determining the etiology of each disease. Prior to its discovery, scientists used more routine methods - crops, cultivation, serology, etc.

Of course, for bacterial pathogens they are effectively used so far, but pathogenic microorganisms such as viruses, mycoplasmas and chlamydia are a particular problem because of the complexity of their growth on an artificial environment. In addition, the first of these are in a state of permanent mutation, which further complicates the task for the medical profession. But PCR diagnostics of infections is universal. It allows you to determine in the biomaterial the most unique in any microbe - its genetic code, more precisely, nucleic acids. This makes it possible to identify the type of pathogen with 100% accuracy, so that it is possible to prescribe the correct treatment as soon as possible without harming the normal microflora with broad-spectrum antibiotics. Thus, PCR diagnostics of infections has found its effective application in medicine, allowing to detect and identify the virus in any biopsy or human analysis: blood, saliva, cerebrospinal fluid or scrapes.

The essence of the method

This method is based on the multiple build-up of nucleic acids in the biomaterial until a full-length strand of the genome or even a whole microbial particle is formed. Therefore, in the first place it was used in the diagnosis of hepatitis viral etiology, STD, HIV, CMV, HPV and tuberculosis. There is also a separate analysis - PCR diagnostics for 12 infections, including the most common sexually transmitted diseases. Thus, this method can reveal chlamydia, candida, CMV, HPV, gardnerella, herpes simplex virus, Trichomonas, Neisseria, mycoplasma and ureaplasma. There are also 5 and 6 component PCR diagnostics of infections. The price for such analyzes remains high enough to use it universally and routinely (about 2000 rubles).